Explore the Fascinating World of the Lithium Chloride Precipitation

Lithium chloride precipitation is widely used for separating RNA from solution.

Here's the breakdown of the process:

1. Sample preparation: Start with a sample containing RNA, such as in-vitro transcript mixture.

2. Lithium chloride addition: Add an appropriate volume of concentrated lithium chloride solution to the RNA solution.

3. Incubation: After adding lithium chloride, the solution is incubated at a low temperature, generally around -20°C. This causes RNA molecules to precipitate out of the solution.

4. Centrifugation: After incubation, the solution is centrifuged at high speed. This separates the precipitated RNA from the rest of the solution, which contains impurities.

5. Washing: Once centrifugation is complete, carefully remove the supernatant but without interfering with the RNA precipitation. Wash the RNA particles with cold ethanol or isopropyl alcohol to remove any remaining impurities.

6. RNA resuspension: After washing, the RNA precipitate is briefly air-dried and then re-suspended in an appropriate buffer, such as TE buffer (Tris-EDTA buffer) or distilled water. Gentle pipetting or eddy currents may be necessary to adequately resuspend the RNA.

7. Quantification and analysis: Finally, the isolated RNA is quantified by ultraviolet spectroscopy or fluorescence method. This determines the concentration and purity of the RNA sample, which is important for downstream applications such as LNP encapsulation, and cell transfection.

Yaohai Bio-Pharma is skilled in using the lithium chloride precipitation method in the production process because it is simple, low-cost, and produces high-quality RNA suitable for a variety of molecular biology applications. However, it is important to optimize conditions (e.g., lithium chloride concentration, incubation time, centrifugation speed) to achieve the best results for a particular sample.

Relied on our powerful and proprietary RNASci platform, we also provide bespoke RNA synthesis services, as well as mRNA (up to 10 kb), circRNA (up to 4 kb), and LNP catalog products. We have established a complete set of mRNA/circRNA synthesis technologies to provide high-quality RNA, such as sequence design and optimization, IVT, circularization, purification, and LNP encapsulation. All products are released under the stringent quality control standards.

Yaohai Bio-Pharma is the first and largest biologics CRDMO dedicated to microbial expression systems in Greater China. We offer end-to-end one-stop tailored solutions or standalone services ranging from RNA synthesis, strain engineering, and construction, upstream and downstream process development, to GMP or non-GMP level drug substance manufacturing, and fill & finish services for diverse modalities, such as mRNA, circRNA, plasmid RNA, recombinant proteins, peptides, enzymes, single domain antibodies (sdAbs) and VLPs. We are committed to meeting global customers' clinical and commercial needs in biological drugs, vaccines, and diagnostics for both human and veterinary use.

Yaohai Bio-Pharma is also actively seeking institutional or individual global partners and offers the most competitive compensation in the industry. If you have any questions, please feel free to contact us: [email protected]