| Service Details |
Unit Operations |
Parameters |
E. coli fermentation
|
Shake Flask |
Media compositions, antibiotic, inoculum volume, growth temperature, and etc. |
|
Fermentor (7 L)
|
Antibiotic, inoculum volume, growth temperature, pH, dissolved oxygen (DO), feeding mode (e.g., fed-batch), feeding media, fermentation time, and etc.
|
| Cell harvest |
Microfiltration |
Standard E. coli harvesting process |
| Cell lysis |
Cell resuspendion, alkaline lysis, neutralization |
Alkali ratio, lysis time |
| Clarification |
Filtration |
Crude filtration, 20 µm and a 5 µm filter train (prefered) |
| Concentration |
Ultrafiltration, tangential flow filtration |
Pore size (or NMWC), Transmembrane pressure (TMP), Flux, filtrate volume to membrane area ratio |
| Three-step chromatography |
Size exclusion chromatography (SEC), or gel filtration chromatography (GFC) |
Column length and diameter, buffer composition, injection volume, mobile phase composition (adsorption and desorption), pH, flow rate, equilibration, binding, washing, and elution conditions.
|
| Affinity chromatography (AF) |
| Anion exchange chromatography (AEX) |
| Concentration |
Ultrafiltration, tangential flow filtration |
Pore size (or NMWC), Transmembrane pressure (TMP), Flux, filtrate volume to membrane area ratio |
| More Biologics Purification Process Solutions |
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