The Significance of Strain Engineering
Strain engineering aims to make the expression host with characteristics of expectations, which is necessary for biologics CMC development and determines the quality and consistency of the product and manufacturing process.
Keywords: strain genetic engineering and screening, protein expression strain construction, microbial cell engineering, bacteria strain engineering, yeast strain engineering, prokaryotic microorganism construction, eukaryotic microorganism engineering, engineered strain
Application: human medicine, animal medicine, vaccine, synthetic biology, recombinant large molecule biologics, biological reagent
Strain Engineering Services of Yaohai Bio-Pharma
Benefiting from over 10 years of CDMO experience in microbial biologics, we have accumulated rich experiences in the engineering and selection of various types of plasmid vectors, and microbial hosts (bacteria, yeast). We can find the best expression system for your unique biologics.
We collected various microbial hosts with clear sources and standard CoA, including
Bacteria: Escherichia coli (E. coli)
Yeast: Pichia pastoris (P. pastoris), Saccharomyces cerevisiae (S. cerevisiae), Hansenula polymorpha (H. polymorpha)
Service Details
|
Services
|
Service Details
|
Minimum Timeline/Day
|
Deliverables
|
|
Strain Engineering
|
Gene knock-out, or Gene deletion
|
TBD
|
Strain engineering report
|
|
Gene knock-in, or Gene insertion
|
|
Strain Construction (E. coli)
|
Plasmid transformation (multi-hosts)
|
5
|
COA of commercial hosts,
Strain construction report
|
|
PCR verification
|
|
Strain purification
|
|
Strain preservation
|
|
Strain Construction (Yeast)
|
Preparation of competent yeast cells
|
10
|
|
Plasmid linearization
|
|
Electro transformation
|
|
Resistance/Nutritional deficiency screening
|
|
Strain purification
|
|
Strain preservation
|
|
Suitable Host Screening
|
Plasmid or Genome DNA extraction
|
15-20
|
Host screening process,
Testing report of engineered strains
|
|
PCR verification, restriction enzyme digestion
|
|
Target gene sequencing
|
|
Screening of high-expression hosts
|
|
Screening of hosts with genetic stability
|
|
Strain preservation
|
Case Study
Engineered Hansenula polymorpha improved HPV L1 VLP expression yield.
We have established yeast Hansenula polymorpha (H. polymorpha) engineering and screening platform for recombinant proteins/peptides expression. Firstly, we generated H. polymorpha protease-deficiency strains by targeted gene deletion. Secondly, a vector carrying the HPV L1 gene was transformed into the competent H. polymorpha cell. Finally, we screen the best clone from hundreds of options, with genetically stability and high-producing properties.
Our Service Features
We have demonstrated expertise in various microbial hosts, e.g., Escherichia coli (E. coli) DH5α, TOP10, Trans10, BL21; Pichia pastoris (P. pastoris) SMD1168H, X-33, GS115, PichiaPink strain1/2/3/4; Saccharomyces cerevisiae (S. cerevisiae) and Hansenula polymorpha (H. polymorpha).
We are experienced with E. coli periplasmic secretion, soluble and inclusion body expression, as well as yeast intracellular or secretion expression.
We add no antibiotics or add the antibiotics under regulatory guidance.
We establish a high throughput screening platform to screen high-producing and stable strains from over one hundred candidate strains.
We make sure that the process of strain engineering and screening is traceable, under Good Data and Record Management.
EN
AR
HR
CS
DA
NL
FI
FR
DE
EL
IT
JA
KO
NO
PL
PT
RO
RU
ES
SV
IW
ID
LV
LT
SR
SK
SL
UK
VI
ET
HU
TH
TR
FA
AF
MS
BE
MK
UR
BN
