Carboxy-terminal (C-terminal) sequence analysis is used for direct confirmation of the C-terminal sequence, C-terminal modification types and C-terminal variation (e.g., C-terminal lysine deletion/ clipping) of native and recombinant proteins. Yaohai Bio-Pharma offers Liquid Chromatography-Mass Spectrometry (LC-MS) based C-terminal sequence testing and data analysis services for your target protein, to speed up your research on protein structure.
We have been involved in the Protein Structural Characterization of various large molecules, including Recombinant Subunit Vaccines, Nanobodies/VHHs/ Single-domain Antibodies (sdAbs), Antibody Fragments, Hormones/Peptides, Cytokines, Growth Factors (GF), Enzymes, Collagens, etc.
Regulatory Requirements for C Terminal Sequencing
According to the ICH Q6B guidelines, it is necessary to confirm the sequence of your protein and examine the termini for any variation that may exist.
C Terminal Sequencing Methods
| Analysis |
Methods |
| C-terminal sequencing |
Liquid Chromatography-Mass Spectrometry (LC-MS) after digestion |
Methods
1. Enzymatic digestion
2. LC-MS analysis
3. Data analysis
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