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Protein Structure Characterization

Protein Structure Characterization

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Microbial CDMO

Protein Structural Characterization

Proteins consist of amino acid chains that interact with each other to form a quaternary structure. The structure of proteins can be primary, secondary, tertiary, or quaternary, and is directly related to their function. Yaohai Bio-pharma offers structure characterization services aligned to ICH Q6B to fully characterize the primary and higher-order structure of protein molecules.

We have been involved in the Protein Structural Characterization of various of large molecules, including Recombinant Subunit Vaccines, Single-domain Antibodies (sdAbs)/VHHs, Antibody Fragments, Hormones/Peptides, Cytokines, Growth Factors (GF), Enzymes, Collagens, etc.

Protein Structural Characterization

Physicochemical Characterization

Primary Amino Acid Sequencing

Higher Order Structure

Post-Translational Modification (PTM)
Analysis Methods
Analysis Methods
Molecular Weight Liquid Chromatography-Mass Spectrometry (LC-MS) / Liquid Chromatography/Electrospray Mass Spectrometry (LC/ES-MS) of intact, reduced and N-deglycosylated proteins
Size Variant Analysis LC-MS, Size Exclusion Chromatography-Mass Spectrometry (SEC-MS), Dynamic Light Scattering (DLS), Analytical Ultracentrifugation (AUC), Size Exclusion Chromatography-Multi Angle Light Scattering (SEC-MALS)
Charge Variant Analysis LC-MS, Ion Exchange Chromatography-Mass Spectrometry (IEX-MS)
Thermal stability (Tm) Differential Scanning Calorimeter (DSC)
Extinction Coefficient Amino acid analyzers
Peptide Mapping LC-MS after protease digestion
Protein Sequence Coverage LC-MS after one-three digestion
N-terminal Sequencing LC-MS after digestion, determined by peptide mappingEdman degradation
C-Terminal Sequencing LC-MS after digestion
Disulfide Bonds LC-MS after digestion
Free Sulfhydryl Groups Ellman’s assay
Amino Acid Oxidation LC-MS, Reverse Phase High Performance Liquid Chromatography (RP-HPLC) or Hydrophobic Interaction Chromatography-High Performance Liquid Chromatography (HIC-HPLC) and mass spectrometry (MS)
Amino Acid Isomerization LC-MS after enzymatic cleavage
Deamidation LC-MS after enzymatic cleavage
N-Terminal Modification LC-MS after digestion
C-Terminal Modification LC-MS after digestion
C-terminal Lysine Clipping LC-MS after digestion
N-glycan Profiling LC-MS of released glycans
N-glycosylation Site LC-MS after enzymatic cleavage
N-glycosylation Site Occupancy LC-MS after deglycosylase and protease (trypsin or Asp-N) digestion
Higher Order Structure Circular Dichroism (CD)Infrared Spectrometer (IR)
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