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Custom mRNA Synthesis

Custom mRNA Synthesis

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IVT RNA

Overview of Custom mRNA Synthesis

mRNA has been widely used in the study of gene or protein functions. Meanwhile, it is common in the development of prophylactic and therapeutic biologics. As a common synthesis strategy for RNA molecules, in vitro transcription (IVT) enables us to obtain customized transcripts efficiently.

Yaohai Bio-Pharma has established a set of mRNA synthesis technologies to provide high-quality RNA, such as sequence design and optimization, IVT, purification, lyophilization and lipid nanoparticle (LNP) encapsulation. All products are released under stringent quality control (QC) standards.

mRNA Applications

With the outbreak of the COVID-19 pandemic in 2020, mRNA technology has advanced by leaps and bounds, and its related research and applications have gained unprecedented development, including prophylactic vaccines for infectious disease prevention, therapeutic oncology vaccines and drugs, tumor therapy, protein replacement therapy, regenerative medicine, and cell and gene therapy (CGT).

mRNA Vaccine mRNA Therapeutics

mRNA Vaccines Against Virus Pathogens

mRNA Therapeutics as Protein Replacement

mRNA Vaccines Against Bacteria Pathogens

mRNA Therapeutics as Antibody Replacement

mRNA Vaccines for Cancer Therapy

mRNA for Gene Editing

 

mRNA as Epigenomic Controller
Services Details
Process Optional Service Service Details Delivery Period (workday) Deliverable
mRNA Sequence Design and Optimization Design and optimization of coding sequences CDS sequence design and codon optimization 1-3 Sequence file
Design and optimization of non-coding sequences Design and optimization of UTR, polyA sequences
Transcription Template Plasmid Preparation Recombinant plasmid preparation Gene synthesis 7-10
Plasmid amplification and extraction 4
Plasmid linearization and purification
mRNA In Vitro Transcription Co-transcriptional capping (one-step method) In vitro transcription (IVT) / Cap analog 1-2 N/A
Nucleotide modifications (UTP/CTP modifications)
DNA template removal (DNase I)
Enzymatic capping (two-step process) IVT 2-3
Nucleotide modifications (UTP/CTP modifications)
DNA template removal (DNase I)
mRNA purification (lithium chloride/magnetic beads)
Enzymatic capping
mRNA Purification Conventional purification scheme Lithium chloride precipitation 1 mRNA drug substance
Magnetic bead purification
Chromatography column purification scheme Combination of multiple chromatography methods 1-2
Buffer exchange Ultrafiltration 1
mRNA Lyophilization Lyophilization Pre-freezing 2-3 mRNA lyophilized powder
Primary drying (sublimation)
Secondary drying (desorption)
mRNAEncapsulation LNP encapsulation LNP encapsulation 2-3 mRNA-LNPDrug product
Concentration and buffer exchange
mRNA Quality Analysis mRNA drug substance/ lyophilized powder Concentration, purity 1 Test Report
Integrity, capping efficiency, polyA tail distribution 2-5
mRNA-LNP drug product Encapsulation efficiency 1
Particle size and distribution
Surface charge
mRNA Expression Validation 293T cell evaluation Cell plating 4
Transient transfection of cells
Fluorescence signal observation 1-3
Western blot (WB)/ Enzyme-linked immunosorbent assay (ELISA)
Other Customizable Options
5' Cap ▪ Uncapped
▪ Cap0, co-capped
▪ Cap1, co-capped
▪ Cap1, enzymatic capping
5’ UTR/3’ UTR ▪ Nature UTR sequence
▪ Mutant/Engineered UTR sequence
3' PolyA Tail ▪ 100A ~120A Tail (recommended)
▪ Segmented polyA tail
▪ Other Custom tails
Modified nucleosides ▪ Unmodified bases
▪ Pseudouridine (Ψ)
▪ N1-Methylpseudouridine (N1Ψ)
▪ 5-methylcytosine (m5C)
▪ 5-methyluridine (m5U)
▪ 5-methoxyuridine (5moU)
▪ 2-thiouridine (s2U)
▪ 2′-O-methyl-U
▪ Other modifications
Our Features

Integrated Service Flow

Provide a series of services from front-end sequence design to back-end mRNA preparation, quality control and expression validation.

International Cutting-edge Sequence Design and Optimization

Professional mRNA sequence design and optimization facilitate efficient mRNA expression.

Diversified Nucleotide Modifications

Effectively increase mRNA expression and reduce mRNA adverse immune responses.

Mature Purification Platform

By combining a conventional and self-designed purification method, mRNA samples are obtained with high purity

Complete QC Platform

Enrich QC options to meet the requirements of routine tests, such as concentration, A260/280 purity, and integrity, as well as high quality control requirements, such as capping efficiency/polyA distribution.

Fast Delivery

Customized mRNA can be delivered in as fast as 7 days except for outsourced sequence synthesis.

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