In order to improve the stability of circRNA and avoid losses in storage and transportation, Yaohai Bio-Pharma can provide circRNA lyophilization services.
The circRNA drug substance (DS) is freeze-dried and stored or transported in the form of lyophilized powder, which significantly reduces the degradation and loss of circRNA.
Services Details
| Process | Optional Service | Service Details | Delivery Period (Day) | Deliverables |
| circRNA lyophilization | Sample Filling | Filling | 2-3 | circRNA lyophilized powder |
| Lyophilization | Pre-freezing |
| Primary drying (sublimation) |
| Secondary drying (desorption) |
| circRNAquality control | Reconstitution of lyophilized powder | Reconstitution/resuspension | - | Test report |
| Solubility of lyophilized powder | Appearance inspection | - |
| Concentration | Ultraviolet (UV) spectrophotometry | 0.5 |
| Purity | Agarose gel electrophoresis (AGE)/E-gel |
| Enzyme-linked immunosorbent assay (HPLC)-optional | 1 |
Our Features
- Mature lyophilization process
The quality attributes of samples before and after lyophilization are consistent, with good repeatability.
- Homogeneous quality properties
circRNA samples before and after lyophilization successfully express the target protein.
circRNA lyophilized powder is easy to store and transport.
Case Study
An E-gel electrophoresis assay is performed for circRNA before and after lyophilization to analyze the integrity and purity, respectively. The results show that there is no significant difference between the circRNA bands before and after lyophilization, and the detection results of the samples after lyophilizationwere reproducible.
Identify of eGFP circRNA samples before and after lyophilization
The 293T cell evaluation of circRNA samples before and after lyophilization shows that strong fluorescent signals are observed in the eGFP circRNA samples before and after lyophilization, and the target protein is expressed efficiently in vitro.
Expression of eGFP circRNA samples before (left) and after (right) lyophilization
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