In order to improve the stability of mRNA and avoid degradation in storage and transportation, Yaohai Bio-Pharma can provide mRNA lyophilization (freeze-drying) services for customers to freeze-dry the mRNA drug substance (DS) and store or transport it in the form of lyophilized powder.
- Dispensing
- Pre-freezing
- Primary drying
- Secondary drying
Services Details
Process | Optional Service | Service Details | Delivery Period (Day) | Deliverables |
mRNA lyophilization | Filling | Filling | 2-3 | mRNA lyophilized powder |
| Lyophilization | Pre-freezing |
| Primary drying (sublimation) |
| Secondary drying (desorption) |
mRNA quality control | Solubility of lyophilized powder | Resuspension | - | Test Report |
| Appearance inspection | - |
| Concentration measurement | Ultraviolet spectrophotometry (UV) | 0.5 |
| Integrity and purity testing | Agarose gel electrophoresis (AGE) |
| Capillary electrophoresis (CE)-optional | 1 |
Our Features
- Mature Lyophilization Process
Lyophilization has no effect on mRNA integrity.
- Homogeneous Quality Properties
mRNA samples before and after lyophilization can successfully express the target protein.
mRNA lyophilized powder is easy to store and transport.
Case study
Using conventional liposomes, mRNA samples before and after lyophilization are transfected into 293T cells for cellular evaluation. The results show that strong fluorescence signals are observed in the catalog eGFP mRNA sample products before and after lyophilization, indicating that the target protein was efficiently expressed in vitro.
eGFP mRNA samples before (left) and after (right) lyophilization
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