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Manufacturing Process of eGFP mRNA

Nov 21, 2024

The production of eGFP (Enhanced Green Fluorescent Protein) mRNA involves a series of molecular biology techniques to generate a functional RNA molecule that encodes for the expression of the eGFP. In the area of eGFP mRNA, Yaohai Bio-Pharma has accu...

Unlocking the Glow: eGFP as the Star of Reporter RNAs
Nov 18, 2024
Enhanced Green Fluorescent Protein (eGFP), a genetically engineered variant of the naturally occurring Green Fluorescent Protein (GFP), has revolutionized how researchers visualize and track gene expression, protein localization, and cellular interac...
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Nanobody: The Breakthrough on Thrombosis Treatment
Nov 15, 2024
Introduction Factor XII (FXII), a key player in blood coagulation and inflammation, has emerged as a promising therapeutic target. Researchers have developed a nanoantibody (Nb) targeting FXII with high affinity and neutralizing activity. Anti-thromb...
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The Versatile Role of eGFP Reporter RNA
Nov 13, 2024
In the intricate world of molecular biology, fluorescent proteins have emerged as invaluable tools, enabling researchers to visualize and track cellular processes in real-time. Among these, Enhanced Green Fluorescent Protein (eGFP) stands out as a be...
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Gene Therapy Evolution: Novel DNA & Optimization
Nov 12, 2024
In the field of DNA applications, plasmid DNA (pDNA) has always been highly favored due to its exceptional stability, ease of production, storage, and transportation. However, as scientific research continues to advance, a series of novel DNA types, ...
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Championing Reporter RNA Modification Methods
Nov 08, 2024
When choosing which method of reporter mRNA modifications is preferable, there is no definitive answer as each method and reporter gene has its own advantages and applicable scenarios. Here are some common mRNA modification methods and reporter genes...
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Overcoming Challenges in Recombinant Protein Purification
Nov 07, 2024
In recent decades, recombinant proteins have proven crucial in combating various diseases, demonstrating great potential. With advancements in genetic engineering, significant breakthroughs have been made in protein drug research. Technologies like f...
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Investigate the Purity of RNA: Ion Exchange Chromatography
Nov 06, 2024
Ion-exchange chromatography effectively purifies RNA by separating molecules with different charges. Depending on their protonation state at various pH levels, RNA molecules can carry positive or negative charges. This property is utilized in ion exc...
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CVB1-VLP: The Pioneer of Enterovirus VLP Vaccines
Nov 05, 2024
Coxsackievirus B1 (CVB1), a member of the enterovirus B species, is a common cause of acute and chronic myocarditis, dilated cardiomyopathy, and purulent meningitis. However, there is currently no vaccine against CVB1. In June 2023, researchers from ...
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Revolutionizing Discovery in mRNA Targeted Delivery Research
Nov 04, 2024
Today Yaohai Bio-Pharma summarized the latest research results of two mRNA giants in targeted delivery, focusing on the perspective of mRNA sequence design and optimization, and combining publicly available patents and literature. The application of ...
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The Power of Plasmid Purification Technology
Oct 30, 2024
Today, Yaohai Bio-Pharma will introduce a method for purifying Plasmid DNA by ion exchange chromatography. Ion exchange chromatography mainly uses solute molecules to combine with solid phase ion exchangers carrying opposite charges. In the case...
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Revolutionizing DNA Vaccine with Nanocarrier
Oct 29, 2024
DNA vaccine technology holds great potential for treating diseases like cancer, atherosclerosis, and diabetes. It involves delivering DNA-encoding protein antigens into cells to produce specific proteins, which are then presented to the immune system...
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Explore the Fascinating World of the Lithium Chloride Precipitation
Oct 28, 2024
Lithium chloride precipitation is widely used for separating RNA from solution. Here's the breakdown of the process: 1. Sample preparation: Start with a sample containing RNA, such as in-vitro transcript mixture. 2. Lithium chloride addition: Add an...
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Recombinant Protein Production: Engineered E. coli Strains
Oct 25, 2024
Escherichia coli (E. coli) has played a significant role in producing recombinant proteins in the biopharmaceutical industry, serving as the first expression vector for manufacturing biological medicine. E. coli boasts rapid growth, convenient geneti...
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2024 Updated mRNA Purification Methods
Oct 23, 2024
Purification, a crucial step in mRNA production, involves removing impurities such as unreacted chemicals or residual materials. The purification process ensures batch-to-batch consistency of mRNA and lipid nanoparticle (LNP) formulations, removing u...
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The Magic of P. pastoris: Shaping Recombinant Protein Production
Oct 16, 2024
Recombinant proteins, which are proteins expressed in host cells through genetic engineering techniques, have found widespread applications in biomedical research, therapy, and diagnostics. The selection of an appropriate expression system is a cruci...
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DNA Vaccine Production Scale-up Strategies
Oct 14, 2024
Since the initial demonstration of gene expression in vivo through naked DNA injection, DNA vaccines, and gene therapies have advanced significantly. Plasmid DNA is used in both approaches to treat diseases by inducing protein expression. In comparis...
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Nanobodies Meet CAR-T: Shaping the Future of Cancer Therapy
Oct 10, 2024
Chimeric Antigen Receptor T-cell (CAR-T) therapy is an emerging cellular immunotherapy that genetically modifies T-cells to express Chimeric Antigen Receptors (CARs) on their surfaces. These receptors enable T-cells to recognize and specifically targ...
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The Manufacturing Process and Applications of Nanobody
Oct 09, 2024
By targeting specific viruses or structures, engineered antibodies can potentially treat certain high-risk populations. However, similar to monoclonal antibodies, their widespread application is hindered by the high production costs and their large s...
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Plasmid DNA: The Key to Unlocking Cancer's Therapy Code
Sep 27, 2024
Recently, gene therapy has showcased the huge potential in cancer treatment. It can introduce exogenous normal genes into human target cells to repair, replace, or modulate genes within the patient, ultimately achieving therapeutic goals at the ...
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