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Championing Reporter RNA Modification Methods

Nov 08, 2024

When choosing which method of reporter mRNA modifications is preferable, there is no definitive answer as each method and reporter gene has its own advantages and applicable scenarios. Here are some common mRNA modification methods and reporter genes...

Overcoming Challenges in Recombinant Protein Purification
Nov 07, 2024
In recent decades, recombinant proteins have proven crucial in combating various diseases, demonstrating great potential. With advancements in genetic engineering, significant breakthroughs have been made in protein drug research. Technologies like f...
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Investigate the Purity of RNA: Ion Exchange Chromatography
Nov 06, 2024
Ion-exchange chromatography effectively purifies RNA by separating molecules with different charges. Depending on their protonation state at various pH levels, RNA molecules can carry positive or negative charges. This property is utilized in ion exc...
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CVB1-VLP: The Pioneer of Enterovirus VLP Vaccines
Nov 05, 2024
Coxsackievirus B1 (CVB1), a member of the enterovirus B species, is a common cause of acute and chronic myocarditis, dilated cardiomyopathy, and purulent meningitis. However, there is currently no vaccine against CVB1. In June 2023, researchers from ...
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Revolutionizing Discovery in mRNA Targeted Delivery Research
Nov 04, 2024
Today Yaohai Bio-Pharma summarized the latest research results of two mRNA giants in targeted delivery, focusing on the perspective of mRNA sequence design and optimization, and combining publicly available patents and literature. The application of ...
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The Power of Plasmid Purification Technology
Oct 30, 2024
Today, Yaohai Bio-Pharma will introduce a method for purifying Plasmid DNA by ion exchange chromatography. Ion exchange chromatography mainly uses solute molecules to combine with solid phase ion exchangers carrying opposite charges. In the case...
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Revolutionizing DNA Vaccine with Nanocarrier
Oct 29, 2024
DNA vaccine technology holds great potential for treating diseases like cancer, atherosclerosis, and diabetes. It involves delivering DNA-encoding protein antigens into cells to produce specific proteins, which are then presented to the immune system...
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Explore the Fascinating World of the Lithium Chloride Precipitation
Oct 28, 2024
Lithium chloride precipitation is widely used for separating RNA from solution. Here's the breakdown of the process: 1. Sample preparation: Start with a sample containing RNA, such as in-vitro transcript mixture. 2. Lithium chloride addition: Add an...
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Recombinant Protein Production: Engineered E. coli Strains
Oct 25, 2024
Escherichia coli (E. coli) has played a significant role in producing recombinant proteins in the biopharmaceutical industry, serving as the first expression vector for manufacturing biological medicine. E. coli boasts rapid growth, convenient geneti...
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2024 Updated mRNA Purification Methods
Oct 23, 2024
Purification, a crucial step in mRNA production, involves removing impurities such as unreacted chemicals or residual materials. The purification process ensures batch-to-batch consistency of mRNA and lipid nanoparticle (LNP) formulations, removing u...
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The Magic of P. pastoris: Shaping Recombinant Protein Production
Oct 16, 2024
Recombinant proteins, which are proteins expressed in host cells through genetic engineering techniques, have found widespread applications in biomedical research, therapy, and diagnostics. The selection of an appropriate expression system is a cruci...
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DNA Vaccine Production Scale-up Strategies
Oct 14, 2024
Since the initial demonstration of gene expression in vivo through naked DNA injection, DNA vaccines, and gene therapies have advanced significantly. Plasmid DNA is used in both approaches to treat diseases by inducing protein expression. In comparis...
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Nanobodies Meet CAR-T: Shaping the Future of Cancer Therapy
Oct 10, 2024
Chimeric Antigen Receptor T-cell (CAR-T) therapy is an emerging cellular immunotherapy that genetically modifies T-cells to express Chimeric Antigen Receptors (CARs) on their surfaces. These receptors enable T-cells to recognize and specifically targ...
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The Manufacturing Process and Applications of Nanobody
Oct 09, 2024
By targeting specific viruses or structures, engineered antibodies can potentially treat certain high-risk populations. However, similar to monoclonal antibodies, their widespread application is hindered by the high production costs and their large s...
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Plasmid DNA: The Key to Unlocking Cancer's Therapy Code
Sep 27, 2024
Recently, gene therapy has showcased the huge potential in cancer treatment. It can introduce exogenous normal genes into human target cells to repair, replace, or modulate genes within the patient, ultimately achieving therapeutic goals at the ...
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Polypeptides Drugs: The Future of Biomedicine
Sep 26, 2024
The market for polypeptide drugs is experiencing rapid growth in the biomedical sector, with an annual compound growth rate of 8.5% globally from 2020 to 2030, expected to reach US$141.8 billion by 2030. Polypeptide drugs are produced via methods lik...
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Yeast: Key Player in VLP Vaccine for Polio
Sep 25, 2024
Poliomyelitis (polio) is caused by a virus, with symptoms including fever, upper respiratory discomfort, and limb paralysis. The disease is preventable but difficult to cure, and can easily lead to lifelong disability or even death. Thus, vaccines ar...
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Marvelous Fermentation Rate Control Strategies
Sep 24, 2024
Microbial fermentation rate control aims to optimize growth conditions by precisely adjusting key factors such as temperature, pH, dissolved oxygen, and substrate concentration, ensuring that microorganisms are in their optimal physiological state. T...
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Pet's Dedicated Insulin Solution for a Sweet Life
Sep 23, 2024
Diabetes mellitus (DM) is a prevalent disease that affects not only humans but also our beloved pets. As pets' diets improve, their chances of developing diabetes increase due to higher rates of obesity. Based on the database encompassing 2.5 million...
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Harnessing the Power of Fed-batch Fermentation Strategy
Sep 20, 2024
Pichia pastoris is widely used in the production of various heterologous proteins. The High-Cell-Density Fermentation (HCDF) technology, implemented through Fed-Batch feeding, has successfully achieved large-scale production of biopharmaceuticals and...
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