Recombinant proteins, which are proteins expressed in host cells through genetic engineering techniques, have found widespread applications in biomedical research, therapy, and diagnostics. The selection of an appropriate expression system is a crucial factor in the successful expression of recombinant proteins.

Yaohai Bio-Pharma boasts over a decade of experience in yeast strain library construction and production. In this article, Yaohai will elaborate on the details of Pichia pastoris (P. pastoris) expression system of recombinant protein.

P. pastoris as expression system of recombinant protein

P. pastoris, as a methylotrophic yeast, is capable of efficiently utilizing C1 compounds such as methanol, making it an ideal host for the production of recombinant proteins. The process involves introducing a vector carrying the target DNA into the yeast, leveraging the eukaryotic cellular properties of the yeast to express the recombinant protein. The heterologous gene expression system using P. pastoris as the host has developed rapidly in recent years and is recognized as an important and widely accepted tool in molecular biology for the production of recombinant proteins.

Strategy for the P. pastoris expression system

Optimization strategies for P. pastoris fermentation processes

To enhance the effectiveness of fermentation processes, integrating strain manipulations and adopting various strategies during P. pastoris fermentation is crucial. Although commonly used conditions for P. pastoris fermentation exist, optimal conditions are highly dependent on the desired product, the genetic composition employed, and whether product regulation is achieved through a constitutive or inducible promoter. Key factors for successful improvement, optimization, and simulation in P. pastoris include the adjustment of fermentation parameters, selection of operational modes, and utilization of mathematical models. Optimizing the fermentation process involves precisely selecting parameters such as temperature, pH, medium composition, and dissolved oxygen, as well as adopting suitable operational modes like continuous & fed-batch fermentation. Furthermore, genome-scale metabolic models and mathematical methods can be leveraged to refine the optimization of the fermentation process.

Conclusion

Compared to bacteria, P. pastoris, as a eukaryotic organism, exhibits unique advantages in the fermentation process. Therefore, P. pastoris is recognized as a major microbial platform for the production of recombinant proteins. To achieve the high yield of the desired protein, process optimization is necessary.

The efficient and rapid development of cell banking is crucial to the product launch process. If you need P. pastoris or other yeast strains construction, Yaohai can provide you with extensive microbial cell banking construction experience, professional regulatory guidance, as well one-stop CDMO services. We have designed a reasonable development roadmap that minimizes the CMC development cycle of recombinant biological products while avoiding risks.

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