Plasmid DNA is an important vector in the field of cell and gene therapy (CGT), which can be used as naked plasmid DNA therapeutics, DNA vaccines, and starting materials for viral vector or mRNA production. Based on a microbial R&D platform, Yaohai Bio-Pharma offers customized plasmid preparation services dedicated to meeting unique objectives. We are experienced in Escherichia coli (E. coli) strain engineering, fermentation, and plasmid purification.
Keywords: plasmid extraction, plasmid purification, poly(A)-plasmid preparation, help plasmid production
Application: cell and gene therapy (CGT), DNA vaccine, mRNA production, saRNA preparation, AAV packaging, virus-vector package
Customized Options
| Modality |
Plasmid DNA |
| Expression system |
E. coli DH5α, TOP10, Trans10, etc. |
| Length of plasmid |
~10,000 nt (10 kb) |
| Deliverables |
Supercoiled plasmid purity: 80%, 90%, 95%
Quantity: 0.2~5 g
|
| Analysis methods |
Content: Ultraviolet (UV, A260)
Purify: UV (A260/A280) and Agarose gel electrophoresis (AGE)
Optional: capillary electrophoresis (CE), high performance liquid chromatography (HPLC)
|
| Residual impurities |
Host cell proteins (HCP), host cell DNA(HCD), endotoxin (optional) |
Service Details
| Services |
Service Details |
Minimal Timeline(workday) |
| Gene synthesis |
Gene synthesis |
7~10 days |
| Plasmid/vector construction |
Cloning into plasmid/vector |
2 days |
| Plasmid transformation |
| Polymerase chain reaction (PCR) verification, restriction enzyme digestion, gene sequencing |
3 days |
| Plasmid amplification in E. coli |
Cultivation in shake flask |
3 days |
| Cultivation in 7L-Fermenter |
4 days |
| Plasmid purification |
5 days |
| QC by UV, AGE, and others |
Additional Services (Optional)
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