DNA vaccine technology holds great potential for treating diseases like cancer, atherosclerosis, and diabetes. It involves delivering DNA-encoding protein antigens into cells to produce specific proteins, which are then presented to the immune system. While this technology can elicit robust immune responses, it faces challenges such as nucleic acid degradation and delivery issues. Various methods, including mechanical and non-mechanical, can enhance DNA delivery, but each has its drawbacks. Therefore, research has focused on designing non-viral vectors, particularly nanocarriers, to improve delivery and efficacy.

In the field of nanobody technology, Yaohai Bio-Pharma boasts extensive experience in nanobody research and production, supported by a mature technical platform, advanced production equipment, and groundbreaking technology in the comprehensive expression system for nanobodies. Regarding our experience, Yaohai will elaborate how Nanoparticles (NPs) improve the DNA vaccine.

NPs as delivery vectors can protect the payload from degradation during transport to target cells. Nanoparticle delivery systems enhance the immunogenicity of DNA payloads, minimize toxicity, boost DNA uptake and nuclear entry, and improve overall antigen-specific immune responses.

Polymer Nanoparticles

Polymer nanoparticles, made of materials like chitosan, PLA, PGA, and PLGA, are safe and versatile in DNA vaccine delivery. They protect DNA from degradation but have limited transfection efficiency.

Lipid Nanoparticles

Lipid nanoparticles show high transfection and encapsulation efficiencies but have toxicity and stability issues. Mixed lipid-polymer nanoparticles improve these aspects.

Lipopolyplexes

Combining polymer materials with lipid-based delivery systems, lipopolyplexes leverage the synergistic benefits of both. Compared to other platforms using only one material, lipopolyplexes exhibit higher transfection efficiency and lower cytotoxicity.

Inorganic Nanoparticles

Inorganic nanoparticles, including gold and iron, offer strong functionalization and biocompatibility. SPIONs enable precise drug delivery with an external magnetic field.

Virus-Like Particles (VLPs)

VLPs resemble viruses but lack genetic material. They are excellent adjuvants, inducing strong immune responses and being easy to functionalize.

Protein-Based Nanoparticles

Protein-based nanoparticles are biocompatible and biodegradable but have low DNA loading capacity and lack cell specificity. Cationic proteins like protamine can be complex with DNA for nuclear transport.

Yaohai Bio-Pharma is also actively seeking institutional or individual global partners and offers the most competitive compensation in the industry. If you have any questions, please feel free to contact us: [email protected]