The products obtained by in vitro cyclization and RNase R enrichment require further removed from in vitro transcription (IVT), including unconsumed substrates in cyclization reaction, reaction by-products and nicked RNA.
Yaohai Bio-Pharma has mature LiCl precipitation, magnetic bead purification, self-developed purification and other diversified purification solutions to effectively remove various impurities and prepare highly pure circRNA.
- LiCl precipitation method
Purification solution for small amounts of circRNA for cell transfection and as part of animal experiments.
- Magnetic bead purification method
Purification solution for small amounts of circRNA for cell transfectionand as part of animal experiments.
- Yaohai Bio-Pharma purification solution
Application scenarios with higher quality；
Stable and scalable purification process to meet GMP production requirements.
Services Details
| Process |
Optional Service |
Service Details |
Delivery Period (Day) |
Deliverables |
| circRNApurification |
Conventional purification solution |
Lithium chloride precipitation |
1 |
circRNA drug substance |
| Magnetic bead purification |
| Highly pure circRNA purification |
Preparative high-performance liquid chromatography (HPLC) |
1-2 |
| Buffer exchange |
Ultrafiltration |
1 |
| circRNAquality control |
Concentration /purify |
Ultraviolet (UV) spectrophotometry |
0.5 |
Test report |
| Purity |
Agarose gel electrophoresis (AGE)/E-gel |
| HPLC-optional |
1 |
Our Features
- A variety of purification options
Application scenarios can be achieved.
circRNA with a purity of more than 90%;
- Stringent control of RNase
Through stringent control of RNases in the experimental environment and consumables, RNA degradation is effectively prevented.
Case Study
Yaohai Bio-Pharma has established a mature circRNA purification solutionthat can effectively remove various process-related impurities.
The circular RNA products purified by the conventional solution are still obviously mixed with nicked RNA and introns.
After the purification solution developed by Yaohai Bio-Pharma, various linear RNA impurities, such as nicked RNA and introns, can be successfully removed.
circRNA Expression Validation
Using a conventional liposome, purified eGFP circRNA is transfected into 293T cells, and a fluorescence signal is observed after 24 h and continues to be enhanced at 48 h. The fluorescence signal can still be detected on the 7th day and the 14th day after transfection.
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