Firefly luciferase saRNA expresses luciferase protein, originally isolated from the firefly, Photinus pyralis. This protein emits bioluminescence in the presence of the substrate, luciferin. Firefly luciferase saRNA is commonly used as positive control in saRNA transfection and delivery system development. Firefly luciferase saRNA can also be utilized in mammalian cell culture to measure both gene expression and cell viability.
Yaohai Bio-Pharma offers unmodified Firefly luciferase saRNA, with Cap1 structure, optimized codons, engineered UTR and 110nt polyA tail, to improve saRNA stability and translation efficiency.
Product
Firefly luciferase saRNA, Cap1, poly(A) tail, unmodified mRNA
Product Details
| Product |
Firefly luciferase saRNA
|
| Cat. No |
saP003 |
| RNA Content |
100 µg~10 mg (OD260) |
| Purity |
A260/A280 |
| Identify and Purity |
Agarose Gel Electrophoresis (AGE) |
| 5' Cap |
Cap1 |
| 3’ polyA tail |
110±5 nt |
| Replicon |
Replicase enzyme encoding sequence of Alphaviruses |
| Base Modification |
Unmodified, Modified |
| Buffer |
RNase free water (liquid) |
| Shipping |
Ship with dry ice; or under ambient temperature |
| Storage |
● Liquid, at or below -20°C
● Lyophilized powder, at 4°C
|
| Application |
Reporter gene, Positive control
|
Other Customizable Options
| 5' Cap |
● Uncapped
● Cap0, co-capped
● Cap1, co-capped
● Cap1, enzymatic capping
|
| Replicon |
● Replicase enzyme encoding sequence of Alphaviruses
|
| 5’ UTR/3’ UTR |
● Nature UTR sequence
● Mutant/Engineered UTR sequence
|
| 3' PolyA Tail |
● 100A ~120A Tail (recommended)
● Segmented polyA tail
● Other Custom tail
|
| Modified nucleosides |
● Unmodified bases,
● Pseudouridine (Ψ),
● N1-Methylpseudouridine (N1Ψ),
● 5-methylcytosine (m5C),
● 5-methyluridine (m5U),
● 5-methoxyuridine (5moU),
● 2-thiouridine (s2U),
● 2′-O-methyl-U Ÿ Others
● Others
|
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