Description
Cre saRNA expresses Cre recombinase derived from the P1 bacteriophage. Cre recombinase is a member of the integrase family of recombinases, which catalyzes the site-specific recombination event between two DNA recognition sites (LoxP sites). Cre recombinase is a widely used tool in the field of molecular biology. Cre saRNA is commonly used in in vitro and in vivo studies.
As a next-generation mRNA, self-amplifying RNA (saRNA) is designed to achieve enhanced protein expression with a reduced dose than conventional mRNA.
Yaohai Bio-Pharma offers unmodified Cre saRNA, with Cap1 structure, Alphaviruses derived replicon, optimized codons, engineered UTR and 110nt poly(A) tail, to improve saRNA stability and translation efficiency.
Products
Cre saRNA, Cap1, poly(A) tail, unmodified RNA
Product Details
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Product
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Cre saRNA
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Cat. No
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saP004
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RNA Content
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100 µg~10 mg (OD260)
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Purity
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A260/A280
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Identify and Purity
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Agarose Gel Electrophoresis
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5’ Cap
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Cap1
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3’ polyA tail
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110±5 nt
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Replicon
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Replicase enzyme encoding sequence of Alphaviruses
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Base Modification
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Unmodified, Modified
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Buffer
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RNase free water (liquid)
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Shipping
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Ship with dry ice; or under ambient temperature
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Storage
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- Liquid, at or below -20°C
- Lyophilized powder, at 4°C
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Application
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Reporter genes, positive control
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saRNA Preparation
saRNA sequence design → DNA template preparation → in vitro transcription (IVT) → co-transcriptional capping or enzymatic capping → RNA purification → RNA lyophilization, the product is lyophilized powder.
Other Customizable Options
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5' Cap
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- Uncapped
- Cap0, co-capped
- Cap1, co-capped
- Cap1, enzymatic capping
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Replicon
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- Replicase enzyme encoding sequence of Alphaviruses
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5’ UTR/3’ UTR
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- Nature UTR sequence
- Mutant/Engineered UTR sequence
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3' PolyA Tail
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- 100A ~120A Tail (recommended)
- Segmented polyA tail
- Other Custom tail
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Modified nucleosides
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- Unmodified bases,
- Pseudouridine (Ψ),
- N1-Methylpseudouridine (N1Ψ),
- 5-methylcytosine (m5C),
- 5-methyluridine (m5U),
- 5-methoxyuridine (5moU),
- 2-thiouridine (s2U),
- 2′-O-methyl-U
- Others
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