Spike SARS-CoV-2 mRNA expresses spike protein (S protein) of SARS-CoV-2 virus. The S protein on the surface of SARS-CoV-2 is a major antigen and its engagement with human ACE2 receptor plays an essential role in viral entry into host cells. The main candidate for COVID-19 vaccine development is the SARS-CoV-2 spike protein.
Yaohai Bio-Pharma offers unmodified or N1Ψ modified Spike SARS-CoV-2 mRNA, with Cap1 structure, optimized codons, engineered UTR and 110nt polyA tail, to improve mRNA stability and translation efficiency.
Product
Spike SARS-CoV-2 mRNA, Cap1, poly(A) tail, unmodified or modified mRNA
| Product |
Spike SARS-CoV-2 mRNA
|
| Cat. No |
mP005 |
| RNA Content |
100 µg~10 mg (OD260) |
| Purity |
A260/A280 |
| Identify and Purity |
Agarose Gel Electrophoresis (AGE) |
| 5’Cap |
Cap1 |
| 3’ poly(A) tail |
110±5 nt |
| Base Modification |
Unmodified, N1Ψ |
| Buffer |
RNase free water (liquid) |
| Shipping |
Ship with dry ice; or under ambient temperature |
| Storage |
● Liquid, at or below -20°C
● Lyophilized powder, at 4°C
|
| Application |
Antigen gene
|
Other Customizable Options
| 5' Cap |
● Uncapped
● Cap0, co-capped
● Cap1, co-capped
● Cap1, enzymatic capping
|
| 5’ UTR/3’ UTR |
● Nature UTR sequence
● Mutant/Engineered UTR sequence
|
| 3' PolyA Tail |
● 100A ~120A Tail (recommended)
● Segmented polyA tail
● Other custom tail
|
| Modified nucleosides |
● Unmodified bases,
● Pseudouridine (Ψ),
● N1-Methylpseudouridine (N1Ψ),
● 5-methylcytosine (m5C),
● 5-methyluridine (m5U),
● 5-methoxyuridine (5moU),
● 2-thiouridine (s2U),
● 2′-O-methyl-U
● Others
|
mRNA Preparation and Cell Expression
Taking linearized plasmid DNA (pDNA) harboring Spike SARS-CoV-2 mRNA coding genes as IVT template, we obtained pure Spike SARS-CoV-2 mRNA transcripts by co-transcriptional capping and purification, with cap1 structure and N1Ψ modification.
Pure Spike SARS-CoV-2 mRNA (1 μg) mixed with a commercial transfection reagent was transfected into 293T cells in a 96 well plate. And Western blotting (WB) was performed after 24 hours.
Expression of Spike SARS CoV-2 mRNA in 293T cells
EN
AR
HR
CS
DA
NL
FI
FR
DE
EL
IT
JA
KO
NO
PL
PT
RO
RU
ES
SV
IW
ID
LV
LT
SR
SK
SL
UK
VI
ET
HU
TH
TR
FA
AF
MS
BE
MK
UR
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