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mCherry mRNA

  • Description
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mCherry mRNA expresses the fluorescent protein, mCherry, originally derived from DsRed, a protein found in Discosoma sp. mCherry protein displays a monomeric fluorophore when exposed to light with an emissions peak at 610 nm. This mRNA is commonly used as positive control (reporter marker) in mRNA transfection and delivery system development.

Yaohai Bio-Pharma offers unmodified or N1Ψ modified mCherry mRNA, with Cap1 structure, optimized codons, engineered UTR and 110nt polyA tail, to improve mRNA stability and translation efficiency.

Product

mCherry mRNA, Cap1, poly(A) tail, unmodified or modified mRNA

Product Details

Product mCherry mRNA
Cat. No mP002
RNA Content 100 µg~10 mg (OD260)
Purity A260/A280
Identify and Purity Agarose gel electrophoresis (AGE)
5’Cap Cap1
3’ poly(A) tail 110±5 nt
Base Modification Unmodified, N1Ψ
Buffer RNase-free water (liquid)
Shipping Ship with dry ice; or under ambient temperature
Storage Liquid, at or below -20°C; Lyophilized powder, at 4°C
Application Reporter genes, Positive control

Other Customizable Options

5' Cap

Uncapped

Cap0, co-capped

Cap1, co-capped

Cap1, enzymatic capping

5’ UTR/3’ UTR

Nature UTR sequence

Mutant/Engineered UTR sequence

3' PolyA Tail

100A ~120A Tail (recommended)

Segmented polyA tail

Other custom tail

Modified nucleosides

Unmodified bases,

Pseudouridine (Ψ),

N1-Methylpseudouridine (N1Ψ),

5-methylcytosine (m5C),

5-methyluridine (m5U),

5-methoxyuridine (5moU),

2-thiouridine (s2U),

2′-O-methyl-U

Others

mRNA Preparation and Cell Expression

Taking linearized plasmid DNA (pDNA) harboring mCherry mRNA coding genes as IVT template, we obtained pure mCherry mRNA transcripts by co-transcriptional capping and purification, with cap1 structure and N1Ψ modification. Pure mCherry mRNA (1 μg) mixed with a commercial transfection reagent was transfected into 293T cells in a 96 well plate. And fluorescence photography was performed after 24 hours.

WPS图片(2)

mCherry mRNA Expression in 293T Cells

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