Firefly luciferase (F-Luc) mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. This protein emits bioluminescence in the presence of the substrate, luciferin.
Firefly luciferase mRNA is commonly used as positive control in mRNA transfection and delivery system development. Firefly luciferase can also be utilized in mammalian cell culture to measure both gene expression and cell viability.
Yaohai Bio-Pharma offers unmodified or N1Ψ modified Firefly luciferase mRNA, with Cap1 structure, optimized codons, engineered UTR and 110nt polyA tail, to improve mRNA stability and translation efficiency.
Product
Firefly luciferase mRNA, Cap1, poly(A) tail, unmodified or modified mRNA
Product Details
| Product |
Firefly luciferase mRNA |
| Cat. No |
mP003 |
| RNA Content |
100 µg~10 mg (OD260) |
| Purity |
A260/A280 |
| Identify and Purity |
Agarose gel electrophoresis (AGE) |
| 5’Cap |
Cap1 |
| 3’ poly(A) tail |
110±5 nt |
| Base Modification |
Unmodified, N1Ψ |
| Buffer |
RNase-free water (liquid) |
| Shipping |
Ship with dry ice; or under ambient temperature |
| Storage |
Liquid, at or below -20°C; Lyophilized powder, at 4°C |
| Application |
Reporter genes, Positive control |
Other Customizable Options
| 5' Cap |
● Uncapped
● Cap0, co-capped
● Cap1, co-capped
● Cap1, enzymatic capping
|
| 5’ UTR/3’ UTR |
● Nature UTR sequence
● Mutant/Engineered UTR sequence
|
| 3' PolyA Tail |
● 100A ~120A Tail (recommended)
● Segmented polyA tail
● Other custom tail
|
| Modified nucleosides |
● Unmodified bases,
● Pseudouridine (Ψ),
● N1-Methylpseudouridine (N1Ψ),
● 5-methylcytosine (m5C),
● 5-methyluridine (m5U),
● 5-methoxyuridine (5moU),
● 2-thiouridine (s2U),
● 2′-O-methyl-U
● Others
|
mRNA Preparation and Cell Expression
Taking linearized plasmid DNA (pDNA) harboring F-Luc mRNA coding genes as IVT template, we obtained pure F-Luc mRNA transcripts by co-transcriptional capping and purification, with cap1 structure and N1Ψ modification.
Pure F-Luc mRNA (1 μg) mixed with a commercial transfection reagent was transfected into 293T cells in a 96 well plate. And luciferase assay was performed after 24 hours.
Expression of Firefly luciferase mRNA in 293T cells
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