eGFP mRNA expresses enhanced green fluorescent protein (eGFP), originally derived from the jellyfish, Aequorea victoria. eGFP displays bright green fluorescence when exposed to light in the blue to ultraviolet range, with an emissions peak at 509 nm. eGFP mRNA is commonly used as reporter marker (positive control) in transfection and delivery system development.
Yaohai Bio-Pharma offers unmodified or N1Ψ modified eGFP mRNA, with Cap1 structure, optimized codons, engineered UTR and 110nt poly(A) tail, to improve mRNA stability and translation efficiency.
Product
eGFP mRNA, Cap1, poly(A) tail, unmodified or modified mRNA
Product Details
| Product |
eGFP mRNA |
| Cat. No |
mP001 |
| RNA Content |
100 µg~10 mg (OD260) |
| Purity |
A260/A280 |
| Identify and Purity |
Agarose gel electrophoresis (AGE) |
| 5’Cap |
Cap1 |
| 3’ poly(A) tail |
110±5 nt |
| Base Modification |
Unmodified, N1Ψ |
| Buffer |
RNase-free water (liquid) |
| Shipping |
Ship with dry ice; or under ambient temperature |
| Storage |
Liquid, at or below -20°C; Lyophilized powder, at 4°C |
| Application |
Reporter genes, Positive control |
Other Customizable Options
| 5' Cap |
● Uncapped
● Cap0, co-capped
● Cap1, co-capped
● Cap1, enzymatic capping
|
| 5’ UTR/3’ UTR |
● Nature UTR sequence
● Mutant/Engineered UTR sequence
|
| 3' PolyA Tail |
● 100A ~120A Tail (recommended)
● Segmented polyA tail
● Other custom tail
|
| Modified nucleosides |
● Unmodified bases,
● Pseudouridine (Ψ),
● N1-Methylpseudouridine (N1Ψ),
● 5-methylcytosine (m5C),
● 5-methyluridine (m5U),
● 5-methoxyuridine (5moU),
● 2-thiouridine (s2U),
● 2′-O-methyl-U
● Others
|
mRNA Preparation and Cell Expression
Taking linearized plasmid DNA (pDNA) harboring eGFP mRNA coding genes as IVT template, we obtained pure eGFP mRNA transcripts by co-transcriptional capping and purification, with cap1 structure and N1Ψ modification.
Pure eGFP mRNA (1 μg) mixed with a commercial transfection reagent was transfected into 293T cells in a 96 well plate. And fluorescence photography was performed after 24 hours.
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