mCherry circRNA expresses a fluorescent protein derived from Discosoma sp. mCherry protein displays a monomeric fluorophore when exposed to light with an emissions peak at 610 nm. mCherry circRNA is commonly used as positive control in circRNA transfection and delivery system development.
Product
mCherry circRNA, Permuted intron-exon (PIE) method
Product Details
| Product |
mCherry circRNA
|
| Cat. No |
ciP002 |
|
Synthesis Method
|
Group I intron self-splicing
|
| RNA Content |
100 µg~10 mg (OD260) |
| Purity |
A260/A280 |
| Identify and Purity |
Agarose Gel Electrophoresis (AGE) |
| Buffer |
RNase free water (liquid) |
| Shipping |
Ship with dry ice; or under ambient temperature |
| Storage |
● Liquid, at or below -20°C
● Lyophilized powder, at 4°C
|
| Application |
Reporter gene, Positive control
|
circRNA Preparation and Cell Expression
Taking linearized plasmid DNA (pDNA) harboring mCherry circRNA coding genes as in vitro testing (IVT) template, we obtained pure mCherry circRNA by in vitro transcription, circulation, and purification.
Pure mCherry circRNA mixed with a commercial transfection reagent was transfected into 293T cells in a 96 well plate. And fluorescence photography was performed after 24 hours, 48 hours, 168 hours and 336 hours.
24h 48h 168h 336h
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