Firefly luciferase circRNA expresses a fluorescent protein, originally isolated from the firefly, Photinus pyralis. This protein emits bioluminescence in the presence of the substrate, luciferin. Firefly luciferase circRNA is commonly used as positive control in circRNA transfection and delivery system development. Firefly luciferase can also be utilized in mammalian cell culture to measure both gene expression and cell viability.
Yaohai Bio-Pharma offers pure firefly luciferase circRNA product, with optimized codons and internal ribosome entry site (IRES), to improve firefly luciferase circRNA circulation efficiency, stability and translation efficiency.
Product
Firefly luciferase circRNA, Permuted intron-exon (PIE) method
Product Details
| Product |
Firefly Luciferase circRNA
|
| Cat. No |
ciP003 |
|
Synthesis Method
|
Group I intron self-splicing
|
| RNA Content |
100 µg~10 mg (OD260) |
| Purity |
A260/A280 |
| Identify and Purity |
Agarose Gel Electrophoresis (AGE) |
| Buffer |
RNase free water (liquid) |
| Shipping |
Ship with dry ice; or under ambient temperature |
| Storage |
● Liquid, at or below -20°C
● Lyophilized powder, at 4°C
|
| Application |
Reporter gene, Positive control
|
circRNA Preparation and Cell Expression
Taking linearized plasmid DNA (pDNA) harboring firefly luciferase circRNA coding genes as in vitro transcript (IVT) template, we obtained pure firefly luciferase circRNA by in vitro transcription, circulation, and purification.
Pure firefly luciferase circRNA mixed with a commercial transfection reagent was transfected into 293T cells in a 96 well plate. And luciferase assay was performed after 48 hours.
Expression of firefly luciferase circRNA in293T cells
EN
AR
HR
CS
DA
NL
FI
FR
DE
EL
IT
JA
KO
NO
PL
PT
RO
RU
ES
SV
IW
ID
LV
LT
SR
SK
SL
UK
VI
ET
HU
TH
TR
FA
AF
MS
BE
MK
UR
BN
