Circular RNAs (circRNA) are a class of single-stranded, covalently closed RNA without 5' or 3' ends. This unique feature can prevent circRNA degradation by exonucleases and empower circRNA with high stability and translation efficiency relative to linear mRNA.
circRNA is synthesized by in vitro circulation from a linear RNA precursor. Process- or product-related impurities can lead to an innate immune response, especially linear RNAs (RNA precursors, nicked RNA, excised introns). It is critical to remove linear RNA impurities and obtain high yields of pure circRNA for in vitro/vivo research and therapeutics applications.
Yaohai Bio-Pharma has established a set of circRNA synthesis technologies to provide highly pure circRNA, involving sequence design and optimization, in vitro transcription (IVT), circularization/cyclization, purification preparative high-performance liquid chromatography (HPLC), and lipid nanoparticle (LNP) encapsulation. All products are released under stringent quality control (QC) standards.
Deliverables
| Product |
Customized highly pure circRNA |
| Synthesis Method |
Group I intron self-splicing |
| RNA Content |
100 µg~10 mg (OD260) |
| Length |
50~8,000 nucleotides |
| Purity |
A260/A280 |
| Identify and Purity |
Agarose gel electrophoresis (AGE), High performance lipid chromatography (HPLC) |
| Buffer |
RNase-free water (liquid) |
| Shipping |
Ship with dry ice; or under ambient temperature |
| Storage |
Liquid, at or below -20°C; Lyophilized powder, at 4°C |
| Application |
LNP formulation development, cell-based assays, animal experiment |
Case Studies
We developed a scalable purification process mainly using preparative HPLC, and prepared circRNA with high purity (＞95%).
Fig. Purification and Analysis of circRNA with High Purity (98%)
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