Description
Cre mRNA expresses Cre recombinase derived from the P1 bacteriophage. Cre recombinase is a member of the integrase family of recombinases, which catalyzes the site-specific recombination event between two DNA recognition sites (LoxP sites). Cre recombinase is a widely used tool in the field of molecular biology. Cre mRNA is commonly used in in vitro and in vivo studies.
Yaohai Bio-Pharma offers unmodified or N1Ψ modified Cre mRNA, with Cap1 structure, optimized codons, engineered UTR and 110nt poly(A) tail, to improve mRNA stability and translation efficiency.
Products
Cre mRNA, Cap1, poly(A) tail, unmodified or modified mRNA
Product Details
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Product
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Cre mRNA
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RNA Content
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100 µg~10 mg (OD260)
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Cat. No
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mP0012
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Purity
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A260/A280
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Identify and Purity
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Agarose Gel Electrophoresis (AGE)
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5’ Cap
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Cap1
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3’ poly(A) tail
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110±5 nt
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Base Modification
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Unmodified, N1Ψ
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Buffer
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RNase free water (liquid)
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Shipping
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Ship with dry ice; or under ambient temperature
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Storage
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- Liquid, at or below -20°C
- Lyophilized powder, at 4°C
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Application
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Positive control
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mRNA Preparation
mRNA sequence design → DNA template preparation → in vitro transcription (IVT) → co-transcriptional capping or enzymatic capping → RNA purification → RNA lyophilization, the product is lyophilized powder.
Other Customizable Options
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5' Cap
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- Uncapped
- Cap0, co-capped
- Cap1, co-capped
- Cap1, enzymatic capping
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5’ UTR/3’ UTR
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- Nature UTR sequence
- Mutant/Engineered UTR sequence
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3' PolyA Tail
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- 100A ~120A Tail (recommended)
- Segmented polyA tail
- Other custom tail
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Modified nucleosides
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- Unmodified bases,
- Pseudouridine (Ψ),
- N1-Methylpseudouridine (N1Ψ),
- 5-methylcytosine (m5C),
- 5-methyluridine (m5U),
- 5-methoxyuridine (5moU),
- 2-thiouridine (s2U),
- 2′-O-methyl-U
- Others
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