Description
Cre circRNA expresses Cre recombinase derived from the P1 bacteriophage. Cre recombinase is a member of the integrase family of recombinases, which catalyzes the site-specific recombination event between two DNA recognition sites (LoxP sites). Cre recombinase is a widely used tool in the field of molecular biology. Cre circRNA is commonly used in in vitro and in vivo studies.
Circular RNA (circRNA) is resistant to exonuclease-mediated degradation and is presumably more stable than most linear RNA.
Yaohai Bio-Pharma offers pure Cre circRNA products, with optimized codons and internal ribosome entry site (IRES), to improve circRNA circulation efficiency, stability and translation efficiency.
Products
Cre circRNA, Permuted intron-exon (PIE) method
Product Details
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Product
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Cre circRNA
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Cat. No
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ciP008
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Synthesis Method
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Group I intron self-splicing
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RNA Content
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100 µg~10 mg (OD260)
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Purity
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A260/A280
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Identify and Purity
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Agarose gel electrophoresis (AGE)
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Buffer
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RNase-free water (liquid)
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Shipping
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Ship with dry ice; or under ambient temperature
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Storage
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- Liquid, at or below -20°C
- Lyophilized powder, at 4°C
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Application
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Positive control
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circRNA Preparation
circRNA sequence design → DNA template preparation → in vitro transcription (IVT) → in vitro circulation → circRNA purification → RNA lyophilization, the product is lyophilized powder.
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