Microbial expression systems, like bacteria (Escherichia coli) and yeast (Hansenula polymorpha, Pichia pastoris, Saccharomyces cerevisiae) are widely used in the biopharmaceutical industry. Apart from Vaccine, Peptide, Enzyme, Nano-antibody, Antibody fragment, Growth factor, Cytokine, Antigen, and Collagen, the following proteins can also produced in microbial cells.
Types/Family | Protein | Application |
Albumin | Human serum albumin (HSA) | As an active substance for low albumin levels or as inactive ingredient |
Feline serum albumin (FSA) | Increasing albumin levels and osmotic pressure | |
Canine serum albumin (CSA) | ||
Bovine serum albumin (BSA) | As a standard reagent in experiments | |
Chemokine | Stromal cell-derived factor-1 (SDF-1) | Protecting the heart from ischemia-reperfusion injury; stimulating cardiac angiogenesis |
Heparin-binding growth factor | Midkine | Exerting activities such as angiogenesis and fibrinolysis. |
Osteoblast factor | MEPE fragment | Promoting bone formation and osteoblast proliferation |
Apolipoprotein | Clusterin (CLU), Apolipoprotein A | Clearing cellular debris and apoptosis |
E3 ubiquitin ligase | Parkin protein | Improving Parkinson's symptoms |
Annexin | Annexin 5 | Inhibiting inflammation and endothelial activation as a potential therapeutic for sepsis |
Neurotoxic peptides | Conopeptides, Conotoxins | For pain management, and cosmetics uses (like anti-wrinkle) |
Botulinum toxin | ||
Fusion protein | Human serum albumin (HSA) fused proteins | Improving pharmacokinetic properties and prolonging the half-life of peptides or proteins (mainly as a half-life extension carrier) |
Designed ankyrin repeat proteins (DARPins), like DARPins containing HSA-binding domains | ||
Other fusion proteins | Custom protein |