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Custom Plasmid Preparation Services

Custom Plasmid Preparation Services

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Custom Plasmid Preparation Services

Plasmid DNA is an important vector in the field of cell and gene therapy (CGT), which can be used as naked plasmid DNA therapeutics, DNA vaccines, and starting materials for viral vector or mRNA production. Based on a microbial R&D platform, Yaohai Bio-Pharma offers customized plasmid preparation services dedicated to meeting unique objectives. We are experienced in Escherichia coli (E. coli) strain engineering, fermentation, and plasmid purification.

Keywords: plasmid extraction, plasmid purification, poly(A)-plasmid preparation, help plasmid production

Application: cell and gene therapy (CGT), DNA vaccine, mRNA production, saRNA preparation, AAV packaging, virus-vector package

Customized Options

ModalityPlasmid DNA
Expression systemE. coli DH5α, TOP10, Trans10, etc.
Length of plasmid~10,000 nt (10 kb)
DeliverablesSupercoiled plasmid purity: 80%, 90%, 95% Quantity: 0.2~5 g
Analysis methodsUltraviolet (UV) for QuantityAgarose gel electrophoresis (AGE) for Purify TestingOptional: capillary electrophoresis (CE), high performance liquid chromatography (HPLC)
Residual impuritiesHost cell proteins (HCP), host cell DNA(HCD), endotoxin (optional)

Services Details

ServicesService DetailsMinimal Timeline(workday)
Gene synthesisGene synthesis7~10 days
Plasmid/vector constructionCloning into plasmid/vector2 days
Plasmid transformation
Polymerase chain reaction (PCR) verification, restriction enzyme digestion, gene sequencing3 days
Plasmid amplification in E. coliCultivation in shake flask3 days
Cultivation in 7L-Fermenter4 days
Plasmid purification5 days
QC by UV, AGE, and others

Additional Services (Optional)

Fermentation Process Development

Purification Process Development

HCP, HCD and Endotoxin Removal

Analysis and Testing

Pilot Scale-Up and Large-Scale GMP Manufacturing

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