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Custom Plasmid Preparation Services

Custom Plasmid Preparation Services

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Custom Plasmid Preparation Services

Plasmid DNA is an important vector in the field of cell and gene therapy (CGT), which can be used as naked plasmid DNA therapeutics, DNA vaccines, and starting materials for viral vector or mRNA production. Based on a microbial R&D platform, Yaohai Bio-Pharma offers customized plasmid preparation services dedicated to meeting unique objectives. We are experienced in Escherichia coli (E. coli) strain engineering, fermentation, and plasmid purification.

Keywords: plasmid extraction, plasmid purification, poly(A)-plasmid preparation, help plasmid production

Application: cell and gene therapy (CGT), DNA vaccine, mRNA production, saRNA preparation, AAV packaging, virus-vector package

Customized Options

Modality Plasmid DNA
Expression system E. coli DH5α, TOP10, Trans10, etc.
Length of plasmid ~10,000 nt (10 kb)
Deliverables Supercoiled plasmid purity: 80%, 90%, 95% Quantity: 0.2~5 g
Analysis methods Ultraviolet (UV) for QuantityAgarose gel electrophoresis (AGE) for Purify TestingOptional: capillary electrophoresis (CE), high performance liquid chromatography (HPLC)
Residual impurities Host cell proteins (HCP), host cell DNA(HCD), endotoxin (optional)

Services Details

Services Service Details Minimal Timeline(workday)
Gene synthesis Gene synthesis 7~10 days
Plasmid/vector construction Cloning into plasmid/vector 2 days
Plasmid transformation
Polymerase chain reaction (PCR) verification, restriction enzyme digestion, gene sequencing 3 days
Plasmid amplification in E. coli Cultivation in shake flask 3 days
Cultivation in 7L-Fermenter 4 days
Plasmid purification 5 days
QC by UV, AGE, and others

Additional Services (Optional)

Fermentation Process Development

Purification Process Development

HCP, HCD and Endotoxin Removal

Analysis and Testing

Pilot Scale-Up and Large-Scale GMP Manufacturing

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